Cultivation and Viability Determination of Mycobacterium leprae
نویسندگان
چکیده
Mycobacterium leprae, despite being recognized as a human pathogen over 140 years ago, remains uncultivable in microbiological culture media or in cell culture systems. Although it is now well established that M. leprae prefers cooler temperatures, slightly acidic microaerophilic conditions, and lipids rather than sugars as an energy source, the exact parameters for a defined axenic medium that would support the growth of M. leprae remain elusive.
منابع مشابه
Molecular determination of Mycobacterium leprae viability by use of real-time PCR.
Mycobacterium leprae, the etiological agent of leprosy, is noncultivable on axenic media. Therefore, the viability of M. leprae for clinical or experimental applications is often unknown. To provide new tools for M. leprae viability determination, two quantitative reverse transcriptase PCR (RT-PCR) assays were developed and characterized. M. leprae sodA mRNA and 16S rRNA were used as RNA target...
متن کاملComparison of allergic reaction between paratuberculin P.P.D. heat killed M. paratuberculosis and lepromin in leprosy patients in Iran.
INTRODucnON Leprosy is caused in human by Mycobacterium leprae, since this organisms can not be cultivated in vitro and there is no suitable laboratory animal for the propagation of lepra bacillus, lepromin, usually prepared from the nodule (leproma) of the patient with clinieal form of lepromatosis leprosy, is used for the determination of allergic reactions (FERNANDEZ) and diagnosis of kind o...
متن کاملThe mouse foot-pad technique for cultivation of Mycobacterium leprae.
Although multiplication of Mycobacterium leprae in the foot pads of immune-competent mice is limited, and no leprosy-like lesions are produced in these animals, the mouse foot-pad system represents the first truly useful and reproducible animal model of M. leprae infection. Its employment has enabled research into basic questions with respect to the microbiology of M. leprae, and the epidemiolo...
متن کاملThe Examination and the Determination of the Viability of Mycobacterium Leprae by Electronmicroscopy
The appearance of M. lepraemurium as seen by electronmicro scopy has been described by McFadzean and Valentine (1958-9). Two forms of the bacilli were observed, one the normal form (Fig. 1 ) and the other a degenerate non-viable form (Fig . 2). It was shown that an estimate of the percentage of degenerate forms present gave an indication as to the viability of a suspension. This paper describe...
متن کامل